https://journals.ipsintelligentsia.com/life-science/index.php/ijamb/issue/feed 2025-04-06T16:09:29-06:00 Managing Editor editor@ipsintelligentsia.com Open Journal Systems <p><strong>Journal Summary: </strong><strong>Scope:</strong> Publishes in all areas of Microbiology and Biotechnology. <strong>ISSN:</strong> Print and Online. <strong>Crossref DOI Prefix: </strong>10.54117. <strong>Frequency:</strong> Bimonthly (6 Issues per year). Jan, Mar, May, Jul, Sept, Nov. <strong>Journal model:</strong> Open Access. <strong>Article Processing Charges:</strong> $20 or #25,000. <strong>Abstracting &amp; Indexing:</strong> Google Scholar, Semantic Scholar, Index Copernicus, Crossref, WorldCat. <strong>Type of articles:</strong> All kinds of articles including book of abstracts and conference proceedings. <strong>Review type:</strong> Double-blind peer review. <strong>License type:</strong> CC BY 4.0. <strong>Area of coverage:</strong> Microbiology, medical microbiology, pharmaceutical microbiology, industrial microbiology, biotechnology</p> https://journals.ipsintelligentsia.com/life-science/index.php/ijamb/article/view/46 2025-04-06T16:09:29-06:00 Anthony Obinna Ekesiobi anthonyekesiobi2@gmail.com Cynthia Chioma Onebunne anthonyekesiobi2@gmail.com

<p>Housefly is a domestic pest, causing serious hazard to public health, serve as mechanical vectors to pathogens causing typhoid, cholera, dysentery and others. The use of insecticides is discouraged to manage housefly therefore, alternative management strategies are inevitable. This study was aimed at determining the larvicidal activity of ethanolic leaf extracts of <em>Ocimum gratissimum</em> (scent leaf) on larvae of <em>Musca domestica</em>. Healthy leaf samples of <em>O. gratissimum </em>were collected, washed, stored, shade dried and grinded to fine powder. Whatman filter process and maceration extraction methods were used to get the extracts for the larval toxicity test. Larvae were reared according to the method described by Pavela. Qualitative and quantitative analysis of extracts were performed using standard analytical method.&nbsp; Phytochemical analysis of the plant extracts showed presence of saponins, flavonoid, alkaloid, tannin, steroid and protein, glycosides and anthraquinone. Four different concentrations (100, 250, 500 and 1000 ppm) of the extracts were used. Four replicates were maintained for each concentration and mortality of the larvae was recorded after 24, 48 and 72hours. Moribund and dead larvae were recorded. The findings of this study indicated that there was 11.50 (46.00 %) ; 6.75 (27.00 %)&nbsp; and 2.75 (11.00 %) mortality after 24; 48 and 72 hrs of exposure to the extract respectively. The result showed that the mortality rate was concentration dependent as highest mortality 21(84.00 %) was recorded in 1000 ppm ethanol after 24 hours. The Concentration-response larvicidal bioassay of the extracts have at 72 hours 2.75 (11.00%) mortality at 246.1ppm (LC50) and 6110.8ppm at (LC95). The control which contained only the larvae in distilled water and Quaker oat with no extract, showed no toxicity. This study revealed that tested botanical extracts had significant potential for controlling <em>housefly </em>as alternative to chemical insecticides<em>.</em> Putting the extracts in the form of commercially available insecticide is recommended in further investigation.</p>

2025-04-06T00:00:00-06:00 Copyright (c) 2025 Anthony Obinna Ekesiobi, Cynthia Chioma Onebunne