Structural Elucidation and Antibacterial Evaluation of Natural Products from the Nigric Section of Aspergillus Species against Sorbitol-Positive and -Negative Escherichia coli

Authors

  • I. H. Iheukwumere Department of Microbiology, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
  • C. M. Iheukwumere Department of Applied Microbiology & Brewing, Faculty of Biosciences, Nnamdi Azikiwe University Awka, Nigeria.
  • A. O. Obianom Department of Applied Microbiology & Brewing, Faculty of Biosciences, Nnamdi Azikiwe University Awka, Nigeria.
  • C. H. Nnadozie Department of Microbiology, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
  • U. F. Onwuasoanya Department of Medical Microbiology and Public Health, Faculty of Medical Laboratory Science, NAU.
  • O. T. Oduoye Department of Biotechnology National Centre for Generic Resources and Biotechnology (NACGRAB), Ibadan, Nigeria.
  • V. E. Ike Department of Biology, University of Agriculture and Environmental Sciences Umuagwo, Imo State.
  • O. H. Obiefuna Department of Microbiology, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
  • E. U. Igboanugo Department of Microbiology, Faculty of Applied and Natural Sciences, Legacy University, Okija, Anambra State, Nigeria.
  • C. E. Ejike Department of Medical Microbiology, COOU.
  • O. E. Udeagbara Department of Science Laboratory Technology, Federal Polytechnic, Oko Anambra State.
  • S. C. Ochibulu Department of Microbiology, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
  • N. N. Onyemekara Department of Biology, University of Agriculture and Environmental Sciences Umuagwo, Imo State
  • U. A. Ihenatuoha Department of Environmental Health, Technology, Federal College of Animal Health and Production Technology, NVRI, VOM, Plateau State.
  • N. E. Nwakoby Department of Microbiology, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
  • C. C. Ilechukwu Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.

DOI:

https://doi.org/10.54117/ijnp.v1i1.29

Keywords:

Natural antibiotics, Aspergillus species, Structural elucidation, E. coli inhibition

Abstract

Studies have shown that limited availability, supply shortages and pricing of conventional antibiotics including carcinogenic and mutagenic nature are serious global problems that restrict access to effective treatment for common bacterial infections, and this may not only worsen clinical outcomes but potentially accelerate the development of antibiotic resistance. Hence this study focused on the structural elucidation of antibacterial substances from Mycelia of Negric section of Aspergillus species using natural sources. The fungal isolates were isolated from garden soil samples using standard microbiological techniques. The fungi were grown in a submerged medium prepared from Phoenix dactylifera (PD) fruits (20g), Chrysophyllum albidum (C.A) fruits (10g), Glycine max (GM) peel (10g) and Musa paradisiaca (MP) peel (10g). The antibacterial substances were precipitated, eluted, purified and structurally elucidated using column chromatographic, thin layer chromatographic and C-S-MS techniques respectively. The Aspergillus species isolated were Aspergillus niger strain HUS1 (ANH1), Aspergillus aculeatus strain AN5 (AAA5) and Aspergillus awamori strain DN-SN2 (AAD2). The optical growth and production of antibacterial substances occurred when the pH, Temperature, Carbon Source and nitrogen source were 7.0, 25, and sugar extracted from PD and NOdz respectively. The purified fractions; oleic acid (N1) > 1-docosene (N2) > I-octadecene (N3) > 2,3 furanone-4-hydroxyl (N4) from ANH1 showed significant (P<0.05) inhibitory activities against sorbitol positive and sorbitol negative Escherichia coli; Escherichia coli O157: H7 strain MB4-1 (SECM41) and Escherichia coli HH35 (SECH35). Similarly trans (2-doscenyl) succinic acid (A2) > E-15-hepadecenal (A1) > silane ethyl-trimethoxy (A3) from AAA5 and oleic acid (D1) > hexacosanoic acid (D2) > n-hexadecenoic acid (D3) > 9-octadecenoic acid (Z) methyl ester (D4) from AAD2 also showed significant (P<0.5) pronounced activities against the Sor+ and Sor- Escherichia coli. From this study, the purified fractions from ANH1, AAA5 and AAD2 showed pronounced activities against uropathogenic Escherichia coli and formed the basis of newer antibiotics from natural sources.

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Published

2025-05-24

How to Cite

Iheukwumere, I. H., Iheukwumere, C. M., Obianom, A. O., Nnadozie, C. H., Onwuasoanya, U. F., Oduoye, O. T., … Ilechukwu, C. C. (2025). Structural Elucidation and Antibacterial Evaluation of Natural Products from the Nigric Section of Aspergillus Species against Sorbitol-Positive and -Negative Escherichia coli. IPS Journal of Natural Products, 1(1), 1–12. https://doi.org/10.54117/ijnp.v1i1.29

Issue

Vol. 1 No. 1 (2025): Jun-Dec

Section

Articles

License

Copyright (c) 2025 I. H. Iheukwumere, C. M. Iheukwumere, A. O. Obianom, C. H. Nnadozie, U. F. Onwuasoanya, O. T. Oduoye, V. E. Ike, O. H. Obiefuna, E. U. Igboanugo, C. C. Ejike, O. E. Udeagbara, S. C. Ochibulu, N. N. Onyemekara, U. A. Ihenatuoha, N. E. Nwakoby, C. C. Ilechukwu

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.